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cellamp whole transcriptome amplification kit  (TaKaRa)


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    Structured Review

    TaKaRa cellamp whole transcriptome amplification kit
    Cellamp Whole Transcriptome Amplification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellamp+whole+transcriptome+amplification+kit/CellAmp+Whole+Transcriptome+Amplification+Kit+(Real+Time)%2C+Ver%2E+2/pmc12847941-610-1-6
    Average 94 stars, based on 86 article reviews
    cellamp whole transcriptome amplification kit - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: Genetic diversity of Collaborative Cross mice implicates FFAR3 as a target for ILC2 anti-inflammatory reprogramming.
    Article Snippet: .. The CellAmp Whole Transcriptome Amplification kit (Takara, cat: 3734) and TaKaRa Ex Taq Hot Start Version (Takara, cat: RR006A) were used for RNA-isolation and cDNA preparation. .. Quantitative realtime PCR was carried out with TaqMan Gene Expression assays: Gapdh (Mm99999915) and Ffar3 (Mm02621638_s1) on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) instrument for 45 cycles.

    Article Title: Tubacin alleviate the reproductive toxicity of deoxynivalenol in mouse oocytes and zygotes via strengthening microtubule stability.
    Article Snippet: .. Total RNA was isolated from pooled embryo samples (5 embryos per biological replicate) using the Takara CellAmp Whole Transcriptome Amplification kit (Takara, 3734) following the manufacturer's protocol. .. Quantitative real-time PCR amplification was performed using the Vazyme ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech, Q711) on a QuantStudio 7 Real-Time PCR System (Applied Biosystems).

    Article Title: Inhibition of neddylation disturbs zygotic genome activation through histone modification change and leads to early development arrest in mouse embryos.
    Article Snippet: Post-translational modification and fine-tuned protein turnover are of great importance in mammalian early embryo development.. Apart from the classic protein degradation promoting ubiquitination, new forms of ubiquitination-like modification are yet to be fully understood.. Here, we demonstrate the function and potential mechanisms of one ubiquitination-like modification, neddylation, in mouse preimplantation embryo development.

    Article Title: Genetic diversity of Collaborative Cross mice implicates FFAR3 as a target for ILC2 anti-inflammatory reprogramming
    Article Snippet: .. The CellAmp Whole Transcriptome Amplification kit (Takara, cat: 3734) and TaKaRa Ex Taq Hot Start Version (Takara, cat: RR006A) were used for RNA-isolation and cDNA preparation. .. Quantitative real-time PCR was carried out with TaqMan Gene Expression assays: Gapdh (Mm99999915) and Ffar3 (Mm02621638_s1) on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) instrument for 45 cycles.

    Article Title: Tubacin alleviate the reproductive toxicity of deoxynivalenol in mouse oocytes and zygotes via strengthening microtubule stability
    Article Snippet: .. Total RNA was isolated from pooled embryo samples (5 embryos per biological replicate) using the Takara CellAmp Whole Transcriptome Amplification kit (Takara, 3734) following the manufacturer's protocol. .. Quantitative real-time PCR amplification was performed using the Vazyme ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech, Q711) on a QuantStudio 7 Real-Time PCR System (Applied Biosystems).

    Article Title: Selective abundance of the stemness-promoting cluster miR-290-295 within the adult substantia nigra dopamine neurons is neuroprotective via preservation of protein synthesis
    Article Snippet: .. For subsequent qRT-PCR, the collected cells were used as a template to synthesize cDNA by CellAmp Whole Transcriptome Amplification Kit (Real Time) Ver.2 (Takara Bio, #3734). ..

    Isolation:

    Article Title: Tubacin alleviate the reproductive toxicity of deoxynivalenol in mouse oocytes and zygotes via strengthening microtubule stability.
    Article Snippet: .. Total RNA was isolated from pooled embryo samples (5 embryos per biological replicate) using the Takara CellAmp Whole Transcriptome Amplification kit (Takara, 3734) following the manufacturer's protocol. .. Quantitative real-time PCR amplification was performed using the Vazyme ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech, Q711) on a QuantStudio 7 Real-Time PCR System (Applied Biosystems).

    Article Title: Tubacin alleviate the reproductive toxicity of deoxynivalenol in mouse oocytes and zygotes via strengthening microtubule stability
    Article Snippet: .. Total RNA was isolated from pooled embryo samples (5 embryos per biological replicate) using the Takara CellAmp Whole Transcriptome Amplification kit (Takara, 3734) following the manufacturer's protocol. .. Quantitative real-time PCR amplification was performed using the Vazyme ChamQ Universal SYBR qPCR Master Mix (Vazyme Biotech, Q711) on a QuantStudio 7 Real-Time PCR System (Applied Biosystems).

    other:

    Article Title: Methods of detecting analytes
    Article Snippet: The purification was carried out with an MBS robot (Magnetic Biosolutions), the biotin labelled cDNA was allowed to bind to the Cl-beads for 10 min and was then eluted into 20 μl of water by heating the bead-water solution to 80° C. to break the biotin-streptavidin binding. dA-Tailing by Terminal Transferase After the purification step, 18 μl of each sample was placed into new 0.2 ml PCR tubes and mixed with 22 μl of a polyA-tailing master mix leading to a 40 μl reaction mixture according to manufacturers instructions consisting of lysis buffer (Takara, Cellamp Whole Transcriptome Amplification kit), TdT Buffer (Takara), 1.5 mM dATP (Takara) and TdT Enzyme mix (TdT and RNase H) (Takara).

    Article Title: Methods of detecting analytes
    Article Snippet: The purification was carried out with an MBS robot (Magnetic Biosolutions), the biotin labelled cDNA was allowed to bind to the C1-beads for 10 min and was then eluted into 20 μl of water by heating the bead-water solution to 80° C. to break the biotin-streptavidin binding. dA-Tailing by Terminal Transferase After the purification step, 18 μl of each sample was placed into new 0.2 ml PCR tubes and mixed with 22 μl of a polyA-tailing master mix leading to a 40 μl reaction mixture according to manufacturers instructions consisting of lysis buffer (Takara, Cellamp Whole Transcriptome Amplification kit), TdT Buffer (Takara), 1.5 mM dATP (Takara) and TdT Enzyme mix (TdT and RNase H) (Takara).

    Reverse Transcription:

    Article Title: Inhibition of neddylation disturbs zygotic genome activation through histone modification change and leads to early development arrest in mouse embryos.
    Article Snippet: Post-translational modification and fine-tuned protein turnover are of great importance in mammalian early embryo development.. Apart from the classic protein degradation promoting ubiquitination, new forms of ubiquitination-like modification are yet to be fully understood.. Here, we demonstrate the function and potential mechanisms of one ubiquitination-like modification, neddylation, in mouse preimplantation embryo development.



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